3MTN
Usp21 in complex with a ubiquitin-based, USP21-specific inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-04-28 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54178 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 169.065, 58.235, 133.596 |
| Unit cell angles | 90.00, 121.19, 90.00 |
Refinement procedure
| Resolution | 19.610 - 2.700 |
| R-factor | 0.22042 |
| Rwork | 0.218 |
| R-free | 0.27291 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3i3t |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.197 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 31000 | |
| <I/σ(I)> | 12.64 | 2.08 |
| Completeness [%] | 99.7 | 95.4 |
| Redundancy | 3.5 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.3 | 291 | 11% PEG 4000, 0.1 M SODIUM CITRATE, 0.1 M AMMONIUM ACETATE, 5 MM TCEP, PH 5.3, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 291K |
