3LXJ
Crystal Structure of the Bromodomain of Human AAA domain containing 2B (ATAD2B)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9204 |
| Spacegroup name | P 65 |
| Unit cell lengths | 79.640, 79.640, 204.051 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.810 - 2.330 |
| R-factor | 0.21405 |
| Rwork | 0.212 |
| R-free | 0.25340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | ensemble of 3DAI 2nxb 2oo1 2oss 2ouo 2rfj 3hmh 3d7c 3dwy |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.307 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.9) |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.810 | 2.460 |
| High resolution limit [Å] | 2.327 | 2.340 |
| Rmerge | 0.080 | 0.653 |
| Number of reflections | 31006 | |
| <I/σ(I)> | 10.8 | 2 |
| Completeness [%] | 99.3 | 99.6 |
| Redundancy | 4.9 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 20% isopropanol 0.1M tris pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
