3LTG

Crystal structure of the Drosophila Epidermal Growth Factor Receptor ectodomain complexed with a low affinity Spitz mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2007-04-10
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97928
Spacegroup name	P 21 21 21
Unit cell lengths	73.750, 120.150, 274.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 3.400
R-factor	0.4077
Rwork	0.406
R-free	0.42675
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3I2T and 3LTF; s-dEGFR ectodomain unliganded and complexed with Spitz
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.570
High resolution limit [Å]	3.400	3.400
Number of reflections	28590
<I/σ(I)>	17	2.1
Completeness [%]	99.0	97.2
Redundancy	7	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	294	11% PEG 20,000 0.1M HEPES pH7.4 3% n-propanol, VAPOR DIFFUSION, HANGING DROP, temperature 294K