3LNZ
Crystal structure of human MDM2 with a 12-mer peptide inhibitor PMI (N8A mutant)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2010-01-06 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54 |
Spacegroup name | P 32 1 2 |
Unit cell lengths | 90.544, 90.544, 196.837 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.950 |
R-factor | 0.22186 |
Rwork | 0.220 |
R-free | 0.26687 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3eqs |
RMSD bond length | 0.020 |
RMSD bond angle | 1.846 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 78.413 | 1.980 |
High resolution limit [Å] | 1.949 | 1.950 |
Rmerge | 0.119 | 0.575 |
Number of reflections | 67730 | |
<I/σ(I)> | 23.1 | 4 |
Completeness [%] | 99.0 | 100 |
Redundancy | 8.2 | 8.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 273 | 0.2 M Mg acetate tetrahydrate sulfate, 0.1 M cacodylate trihydrate, 20% PEG 8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 273K |