3KS8
Crystal structure of Reston ebolavirus VP35 RNA binding domain in complex with 18bp dsRNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-12 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 31 |
| Unit cell lengths | 85.685, 85.685, 108.812 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.117 - 2.401 |
| R-factor | 0.2 |
| Rwork | 0.198 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ks4 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.304 |
| Data reduction software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_222)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.100 | 2.380 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Rmerge | 0.061 | 0.254 |
| Number of reflections | 33227 | |
| <I/σ(I)> | 23.47 | 5.03 |
| Completeness [%] | 81.4 | 35.3 |
| Redundancy | 4.3 | 1.43 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295.5 | 365mM ammonium acetate, 100mM trisodium citrate, 18.5% PEG 4000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.5K |
