3HQU

PHD2:Fe:UN9:partial HIF1-alpha substrate complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX10.1
Synchrotron site	SRS
Beamline	PX10.1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-05-08
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.29
Spacegroup name	P 63
Unit cell lengths	110.819, 110.819, 39.615
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.550 - 2.300
Rwork	0.188
R-free	0.22200
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	2g19
RMSD bond length	0.007
RMSD bond angle	1.500
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.068	0.312
Number of reflections	12581
<I/σ(I)>	16.658	3.3
Completeness [%]	99.1	96.2
Redundancy	3.2	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	hanging drop	6.5	298	1.6M AMMONIUM SULFATE, 4% DIOXANE, 0.1M MES PH6.5, ARGON ATMOSPHERE, 20MG/ML PROTEIN WITH 1MM FE(II)SO4, 10MM HIF1A-CODD-OH PEPTIDE, hanging drop, temperature 298K