3H7H

Crystal structure of the human transcription elongation factor DSIF, hSpt4/hSpt5 (176-273)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-10-14
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.283
Spacegroup name	P 21 21 21
Unit cell lengths	41.096, 52.091, 97.099
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.110 - 1.550
Rwork	0.186
R-free	0.22200
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.277
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHELXS
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.550
Number of reflections	28930

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	290	Ammonium sulfate, PEG 3350, Tris/HCl, beta-mercaptoethanol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K