3GZN
Structure of NEDD8-activating enzyme in complex with NEDD8 and MLN4924
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2008-02-16 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97929 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 135.030, 228.715, 229.307 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.360 - 3.000 |
| R-factor | 0.23325 |
| Rwork | 0.230 |
| R-free | 0.28702 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1r4m |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.147 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 49.360 |
| High resolution limit [Å] | 3.000 |
| Rmerge | 0.094 |
| <I/σ(I)> | 15.9 |
| Completeness [%] | 92.3 |
| Redundancy | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 293 | 1.8 M tri-ammonium citrate pH 7.0, 4% (v/v) 1,3 butanediol, temperature 293K |
