3GZN

Structure of NEDD8-activating enzyme in complex with NEDD8 and MLN4924

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	NEDD8-activating enzyme E1 regulatory subunit	polymer	534	60315.1	2	UniProt (Q13564) Pfam (PF00899)	Homo sapiens (human)	Amyloid protein-binding protein 1, Amyloid beta precursor protein-binding protein 1, 59 kDa, APP-BP1, Proto-oncogene protein 1
2	B, D (B, D)	NEDD8-activating enzyme E1 catalytic subunit	polymer	463	51906.2	2	UniProt (Q8TBC4) Pfam (PF00899) Pfam (PF08825)	Homo sapiens (human)	Ubiquitin-like modifier-activating enzyme 3, Ubiquitin-activating enzyme 3, NEDD8-activating enzyme E1C, Ubiquitin-activating enzyme E1C
3	E, F (I, J)	NEDD8	polymer	82	9402.9	2	UniProt (Q15843) Pfam (PF00240)	Homo sapiens (human)	Ubiquitin-like protein Nedd8, Neddylin, Neural precursor cell expressed developmentally down-regulated protein 8, NEDD-8
4	G, H (B, D)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
5	I, J (I, J)	[(1S,2S,4R)-4-{4-[(1S)-2,3-dihydro-1H-inden-1-ylamino]-7H-pyrrolo[2,3-d]pyrimidin-7-yl}-2-hydroxycyclopentyl]methyl sulfamate	non-polymer		443.5	2	Chemie (B39)

Sequence modifications

I, J: 1 - 76 (UniProt: Q15843)

PDB	External Database	Details
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	243248.3
Non-Polymers*	Number of molecules	4
	Total formula weight	1017.9
All*	Total formula weight	244266.1

*Water molecules are not included.