3EQY
Crystal structure of human MDMX in complex with a 12-mer peptide inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-05-27 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 31 |
| Unit cell lengths | 75.627, 75.627, 35.181 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 1.630 |
| R-factor | 0.15551 |
| Rwork | 0.155 |
| R-free | 0.16874 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2z5t |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.746 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0077) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.510 | 1.690 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Rmerge | 0.045 | 0.101 |
| Number of reflections | 27972 | |
| <I/σ(I)> | 64.3 | 20.2 |
| Completeness [%] | 99.9 | 94.8 |
| Redundancy | 5.2 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.1M HEPES, 0.8 M sodium phosphate, 0.8 M potassium phosphate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
