3EGI

Methyltransferase domain of human trimethylguanosine synthase TGS1 bound to m7GpppA (inactive form)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7A
Temperature [K]	130
Detector technology	CCD
Collection date	2006-06-05
Detector	MAR CCD 165 mm
Wavelength(s)	0.9799, 0.9801
Spacegroup name	P 43 21 2
Unit cell lengths	213.893, 213.893, 62.415
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.900 - 2.210
Rwork	0.210
R-free	0.25200
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.291
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	REFMAC (5.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.048
Number of reflections	71001
Completeness [%]	97.0
Redundancy	8.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.1	293	Na-Formate, pH 6.10, VAPOR DIFFUSION, HANGING DROP, temperature 293K