3DO4
Crystal structure of transthyretin variant T60A at acidic pH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-03-06 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9729 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.586, 75.907, 107.267 |
Unit cell angles | 90.00, 93.10, 90.00 |
Refinement procedure
Resolution | 107.200 - 2.400 |
R-factor | 0.20517 |
Rwork | 0.202 |
R-free | 0.26743 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1f41 |
RMSD bond length | 0.027 |
RMSD bond angle | 2.215 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 107.200 | 2.530 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.069 | 0.486 |
Number of reflections | 38055 | |
<I/σ(I)> | 14.3 | 2.9 |
Completeness [%] | 99.6 | 99.8 |
Redundancy | 4 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 4.6 | 298 | 15% PEG 4000, 0.05M NaAcetate, 0.1M AmmoniumAcetate , pH 4.6, VAPOR DIFFUSION, temperature 298K |