3D4B
Crystal structure of Sir2Tm in complex with Acetyl p53 peptide and DADMe-NAD+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 77.4 |
Detector technology | CCD |
Collection date | 2005-07-03 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.99 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.790, 59.735, 106.114 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.900 |
R-factor | 0.1972 |
Rwork | 0.195 |
R-free | 0.22948 |
RMSD bond length | 0.016 |
RMSD bond angle | 3.431 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.950 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.100 | 0.370 |
Number of reflections | 23509 | |
Completeness [%] | 99.4 | 96.54 |
Redundancy | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 292 | PEG 3350, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |