2ZKU
Structure of hepatitis C virus NS5B polymerase in a new crystal form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-10-12 |
Detector | ADSC QUANTUM 210 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 101.990, 102.060, 251.810 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.640 - 1.950 |
R-factor | 0.206 |
Rwork | 0.206 |
R-free | 0.24600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1c2p |
RMSD bond length | 0.005 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.640 | 2.020 |
High resolution limit [Å] | 1.950 | 1.950 |
Number of reflections | 185695 | |
<I/σ(I)> | 15 | 1.54 |
Completeness [%] | 97.0 | 95.2 |
Redundancy | 4.1 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | PROTEIN CONCENTRATION 40MG/ML IN 5MM 2-MERCAPTOETHANOL, 18% PEG 4000, 0.3M NACL, 0.1M SODIUM ACETATE BUFFER PH 5.0, 5MM MERCAPTOETHANOL, pH 5.00, VAPOR DIFFUSION, HANGING DROP, temperature 298K |