2ZC5
Penicillin-binding protein 1A (PBP 1A) acyl-enzyme complex (biapenem) from Streptococcus pneumoniae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 185.704, 50.665, 110.784 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.950 - 3.000 |
| R-factor | 0.2244 |
| Rwork | 0.222 |
| R-free | 0.27600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zc6 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.010 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.950 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.130 | 0.350 |
| Number of reflections | 19127 | |
| <I/σ(I)> | 6.2 | 2.8 |
| Completeness [%] | 88.1 | 91.5 |
| Redundancy | 3.9 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | 0.004-0.006M zinc sulfate, 0.05M MES, pH6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
