2YMD
Crystal structure of a mutant binding protein (5HTBP-AChBP) in complex with serotonin (5-hydroxytryptamine)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-06-02 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 203.565, 140.064, 126.528 |
| Unit cell angles | 90.00, 99.08, 90.00 |
Refinement procedure
| Resolution | 46.903 - 1.960 |
| R-factor | 0.1595 |
| Rwork | 0.159 |
| R-free | 0.20110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xys |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.061 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.900 | 2.190 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.010 | 0.080 |
| Number of reflections | 249028 | |
| <I/σ(I)> | 6.4 | 1 |
| Completeness [%] | 99.6 | 99.6 |
| Redundancy | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 400 MM (NH4)H2PO4 |
