2YCW
TURKEY BETA1 ADRENERGIC RECEPTOR WITH STABILISING MUTATIONS AND BOUND ANTAGONIST CARAZOLOL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2008-05-01 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 89.398, 57.056, 109.542 |
| Unit cell angles | 90.00, 113.52, 90.00 |
Refinement procedure
| Resolution | 44.680 - 3.000 |
| R-factor | 0.2504 |
| Rwork | 0.248 |
| R-free | 0.29495 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vt4 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.129 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.700 | 3.080 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.100 | 0.520 |
| Number of reflections | 17906 | |
| <I/σ(I)> | 9.8 | 1.6 |
| Completeness [%] | 86.0 | 64 |
| Redundancy | 2.4 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.1 | pH 8.1 |
