2Y4I
KSR2-MEK1 heterodimer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-02-12 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 130.565, 130.565, 221.005 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.860 - 3.460 |
R-factor | 0.2327 |
Rwork | 0.230 |
R-free | 0.29340 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 1S9J 2p55 1uwh AND 3C4C |
RMSD bond length | 0.003 |
RMSD bond angle | 0.729 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CaspR |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.650 |
High resolution limit [Å] | 3.450 | 3.450 |
Rmerge | 0.093 | 0.590 |
Number of reflections | 25175 | |
<I/σ(I)> | 10.5 | 2.1 |
Completeness [%] | 99.7 | 100 |
Redundancy | 5.7 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.25 | 293 | 12% (W/V) PEG 3350, 0.2 M NA CITRATE, 10 MM PR ACETATE, BIS-TRIS PROPANE PH 6.25. |