2XRW
Linear binding motifs for JNK and for calcineurin antagonistically control the nuclear shuttling of NFAT4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-02-18 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.230, 94.130, 47.840 |
Unit cell angles | 90.00, 110.74, 90.00 |
Refinement procedure
Resolution | 25.689 - 1.330 |
R-factor | 0.1656 |
Rwork | 0.165 |
R-free | 0.18660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ukh |
RMSD bond length | 0.011 |
RMSD bond angle | 1.357 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.070 | 1.400 |
High resolution limit [Å] | 1.330 | 1.330 |
Rmerge | 0.030 | 0.680 |
Number of reflections | 86996 | |
<I/σ(I)> | 17.45 | 1.98 |
Completeness [%] | 99.2 | 99.3 |
Redundancy | 3.7 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.5 | 18-20% PEG 8000, 100 MM SODIUM CITRATE PH 5.5, 20% GLYCEROL |