2XRW
Linear binding motifs for JNK and for calcineurin antagonistically control the nuclear shuttling of NFAT4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-18 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.230, 94.130, 47.840 |
| Unit cell angles | 90.00, 110.74, 90.00 |
Refinement procedure
| Resolution | 25.689 - 1.330 |
| R-factor | 0.1656 |
| Rwork | 0.165 |
| R-free | 0.18660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ukh |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.357 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.070 | 1.400 |
| High resolution limit [Å] | 1.330 | 1.330 |
| Rmerge | 0.030 | 0.680 |
| Number of reflections | 86996 | |
| <I/σ(I)> | 17.45 | 1.98 |
| Completeness [%] | 99.2 | 99.3 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 18-20% PEG 8000, 100 MM SODIUM CITRATE PH 5.5, 20% GLYCEROL |
