2XMR
Crystal structure of human NDRG2 protein provides insight into its role as a tumor suppressor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6C1 |
| Synchrotron site | PAL/PLS |
| Beamline | 6C1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 86.232, 88.049, 126.907 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.18282 |
| Rwork | 0.180 |
| R-free | 0.23247 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qmq |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.342 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.140 | 0.440 |
| Number of reflections | 63329 | |
| <I/σ(I)> | 11.27 | 1.83 |
| Completeness [%] | 97.0 | 92.2 |
| Redundancy | 4.6 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 16% PEG 8000, 0.2 M CALCIUM ACETATE, 0.1 M MES (PH 6.8 TO 7.1) |
