2X1X

CRYSTAL STRUCTURE OF VEGF-C IN COMPLEX WITH DOMAINS 2 AND 3 OF VEGFR2 IN A TETRAGONAL CRYSTAL FORM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2009-04-20
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 42 21 2
Unit cell lengths	88.733, 88.733, 105.273
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.000 - 3.100
R-factor	0.27292
Rwork	0.266
R-free	0.33391
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2x1w
RMSD bond length	0.006
RMSD bond angle	1.114
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0072)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.000	3.200
High resolution limit [Å]	3.100	3.100
Rmerge	0.100	0.550
Number of reflections	8092
<I/σ(I)>	29.2	7.5
Completeness [%]	99.9	100
Redundancy	17.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.6		100 MM NA-ACETATE BUFFER, PH 4.4-4.8, 50 MM CSCL, 28-32 % (W/V) JEFFAMINE 600