2WZQ

Insertion Mutant E173GP174 of the NS3 protease-helicase from dengue virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	CCD
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	52.420, 87.720, 75.779
Unit cell angles	90.00, 92.90, 90.00

Refinement procedure

Resolution	30.000 - 2.800
R-factor	0.22
Rwork	0.220
R-free	0.28800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vbc
RMSD bond length	0.010
RMSD bond angle	1.305
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.110	0.560
Number of reflections	17435
<I/σ(I)>	8.5	2.3
Completeness [%]	99.9	100
Redundancy	4.1	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1