2WQE

Structure of S155R Aurora-A somatic mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	92
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 41 21 2
Unit cell lengths	85.575, 85.575, 79.949
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.249 - 2.500
R-factor	0.2062
Rwork	0.204
R-free	0.25060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ol6
RMSD bond length	0.007
RMSD bond angle	1.243
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	85.400	2.750
High resolution limit [Å]	2.500	2.500
Rmerge	0.070	0.430
Number of reflections	10725
<I/σ(I)>	8.3	1.8
Completeness [%]	99.1	99.1
Redundancy	7.5	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				17% (W/V) PEG3350, 15% (V/V) GLYCEROL