2W97
Crystal Structure of eIF4E Bound to Glycerol and eIF4G1 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-04-25 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 84.106, 38.201, 93.687 |
Unit cell angles | 90.00, 101.51, 90.00 |
Refinement procedure
Resolution | 28.020 - 2.290 |
R-factor | 0.199 |
Rwork | 0.197 |
R-free | 0.23900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2v8w |
RMSD bond length | 0.011 |
RMSD bond angle | 1.292 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.080 | 0.360 |
Number of reflections | 26638 | |
<I/σ(I)> | 5.7 | 4.3 |
Completeness [%] | 99.6 | 99.7 |
Redundancy | 4.4 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 60% AMMONIUM SULPHATE, 100MM TRIS-HCL PH 8.0, 1% PEG400 |