2VFX
Structure of the Symmetric Mad2 Dimer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-08-16 |
Detector | ADSC CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 109.337, 191.407, 154.307 |
Unit cell angles | 90.00, 90.02, 90.00 |
Refinement procedure
Resolution | 45.000 - 1.950 |
R-factor | 0.209 |
Rwork | 0.208 |
R-free | 0.24700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1klq |
RMSD bond length | 0.011 |
RMSD bond angle | 1.501 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.370 | 1.980 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.070 | 0.550 |
Number of reflections | 223558 | |
<I/σ(I)> | 14.9 | 1.6 |
Completeness [%] | 97.7 | 95.7 |
Redundancy | 2.9 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | VAPOR DIFFUSION, HANGING DROP, 20 DEGREES C. 1 MICROLITER PROTEIN: 3 MG/ML IN 20 MM TRIS, PH 8.0, 50 MM NACL, 2 MM TCEP PLUS 1 MICROLITER RESERVOIR: 19% PEG2000, 16% GLYCEROL, 100 MM TRIS, PH 8.0, 0.3 M MGCL2 |