2VDX
Crystal Structure of the reactive loop Cleaved Corticosteroid Binding Globulin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 297 |
| Detector technology | CCD |
| Collection date | 2007-07-23 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 39.941, 39.740, 112.113 |
| Unit cell angles | 81.94, 84.06, 65.39 |
Refinement procedure
| Resolution | 55.470 - 1.840 |
| R-factor | 0.194 |
| Rwork | 0.192 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qmn |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.272 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.400 | 1.940 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.070 | 0.350 |
| Number of reflections | 51706 | |
| <I/σ(I)> | 8.7 | 1.5 |
| Completeness [%] | 96.0 | 94.1 |
| Redundancy | 2.2 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.4 | 10-20% PEG3350, 0.2M NACL, pH 7.4 |
