2VBL
Molecular basis of human XPC gene recognition and cleavage by engineered homing endonuclease heterodimers
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.612, 69.209, 88.671 |
| Unit cell angles | 90.00, 95.46, 90.00 |
Refinement procedure
| Resolution | 32.920 - 1.800 |
| R-factor | 0.149 |
| Rwork | 0.147 |
| R-free | 0.19700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g9z |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.975 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 32.920 |
| High resolution limit [Å] | 1.800 |
| Rmerge | 0.050 |
| Number of reflections | 45123 |
| Completeness [%] | 95.5 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 4 MG/ML PROTEIN, 35% METHANOL, 0.1M NACACODYLATE PH 6.5 |
