2RAX
Crystal structure of Borealin (20-78) bound to Survivin (1-120)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-23 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.97946 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 145.521, 145.521, 217.429 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.630 - 3.300 |
| R-factor | 0.23896 |
| Rwork | 0.236 |
| R-free | 0.28421 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Survivin 1-142 (truncated to 1-120) + borealin |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.204 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.420 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Number of reflections | 17601 | |
| <I/σ(I)> | 8.4 | 2 |
| Completeness [%] | 98.7 | 98 |
| Redundancy | 5.6 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 292 | 0.5 ul protein plus 0.5 uL well solution consisting of 0.1 M HEPES, pH 7.5; 10% (w/v) PEG 8000; 8 % (v/v) ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
