2R5P
Crystal Structure Analysis of HIV-1 Subtype C Protease Complexed with Indinavir
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-11 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.727, 59.526, 87.384 |
| Unit cell angles | 90.00, 95.56, 90.00 |
Refinement procedure
| Resolution | 27.030 - 2.300 |
| Rwork | 0.212 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.150 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.084 | 0.332 |
| Number of reflections | 21311 | |
| <I/σ(I)> | 8.4 | 3 |
| Completeness [%] | 99.1 | 99.5 |
| Redundancy | 3.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 30 mM citric acid, 1 M NaCl, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
