2QUZ
Crystal Structure of the activating H-RasK117R mutant in Costello Syndrome, bound to Mg-GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.973 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 92.100, 92.100, 116.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 22.330 - 1.490 |
| R-factor | 0.204 |
| Rwork | 0.203 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4q21 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.337 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.030 | 1.580 |
| High resolution limit [Å] | 1.490 | 1.490 |
| Number of reflections | 31034 | |
| <I/σ(I)> | 17.8 | 2.63 |
| Completeness [%] | 99.6 | 97.7 |
| Redundancy | 10.4 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 293 | 37% PEG3350, 50mM TrisHCl pH8, pH 7.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
