2QRV

Structure of Dnmt3a-Dnmt3L C-terminal domain complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	173
Detector technology	CCD
Collection date	2006-04-06
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0, 0.9792, 0.9785,0.9719
Spacegroup name	H 3
Unit cell lengths	401.881, 401.881, 49.689
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.900 - 2.890
R-factor	0.259
Rwork	0.259
R-free	0.28100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2pv0
RMSD bond length	0.009
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.990
High resolution limit [Å]	2.890	2.890
Rmerge	0.058	0.488
Number of reflections	65264
<I/σ(I)>	14.8	1.8
Completeness [%]	100.0	92.1
Redundancy	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	300	The final protein solution CONTAINED ~100 TO 133 micro M COMPLEX IN 20 milli M TRIS/HCL, PH 8.0, 100 milli M NACL, 5 % GLYCEROL, AND 0.1 % MERCAPTOETHANOL. Crystals were obtained with the mother liquor containing 2~5 % PEG 3000, 100 mM Tris/HCl, pH 8.0, 5 % glycerol at 16C, VAPOR DIFFUSION, HANGING DROP
1	VAPOR DIFFUSION, HANGING DROP	8	300	The final protein solution CONTAINED ~100 TO 133 micro M COMPLEX IN 20 milli M TRIS/HCL, PH 8.0, 100 milli M NACL, 5 % GLYCEROL, AND 0.1 % MERCAPTOETHANOL. Crystals were obtained with the mother liquor containing 2~5 % PEG 3000, 100 mM Tris/HCl, pH 8.0, 5 % glycerol at 16C, VAPOR DIFFUSION, HANGING DROP