2QQN

Neuropilin-1 b1 Domain in Complex with a VEGF-Blocking Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-12-06
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.979
Spacegroup name	H 3
Unit cell lengths	214.115, 214.115, 45.465
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.200
R-factor	0.162
Rwork	0.159
R-free	0.20700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1KEX (b1 domain) 2FJH (Fab)
RMSD bond length	0.013
RMSD bond angle	1.427
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.280
High resolution limit [Å]	2.200	2.200
Number of reflections	39195
<I/σ(I)>	14.3	2.2
Completeness [%]	99.0	98
Redundancy	4.4	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		297	25% PEG 1500, 4% tert-butanol, VAPOR DIFFUSION, SITTING DROP, temperature 297K