2QOS
Crystal structure of complement protein C8 in complex with a peptide containing the C8 binding site on C8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-19 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97531 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 59.942, 85.251, 41.899 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.600 - 1.810 |
| Rwork | 0.204 |
| R-free | 0.24400 |
| Starting model (for MR) | 2ove |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.168 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.870 |
| High resolution limit [Å] | 1.810 | 1.810 |
| Rmerge | 0.063 | 0.277 |
| Number of reflections | 19234 | |
| <I/σ(I)> | 16.2 | 5.2 |
| Completeness [%] | 94.4 | 73.1 |
| Redundancy | 6.3 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 26-28% PEG6K, 0.1M Tris pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
