2QKH
Crystal structure of the extracellular domain of human GIP receptor in complex with the hormone GIP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 93 |
Detector technology | IMAGE PLATE |
Collection date | 2005-08-28 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | H 3 2 |
Unit cell lengths | 84.007, 84.007, 180.947 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.000 - 1.900 |
R-factor | 0.167 |
Rwork | 0.166 |
R-free | 0.18300 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.175 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | DM (5.0) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.098 | 0.538 |
Number of reflections | 19647 | |
<I/σ(I)> | 12.3 | 8.2 |
Completeness [%] | 99.8 | 100 |
Redundancy | 50.1 | 48.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 288 | 100 mM HEPES, 1 M potassium-sodium tartrate, 9% Methyl-beta-cyclodextrin, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 288K |