2QCM
Crystal structure of the orotidine-5'-monophosphate decarboxylase domain (Asp312Asn mutant) of human UMP synthase bound to 6-hydroxymethyl-UMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-04-13 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5419 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 77.378, 116.607, 61.910 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.240 - 1.670 |
R-factor | 0.14213 |
Rwork | 0.141 |
R-free | 0.15927 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.421 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.240 | 1.720 |
High resolution limit [Å] | 1.660 | 1.660 |
Number of reflections | 32717 | |
<I/σ(I)> | 26 | 4.3 |
Completeness [%] | 97.3 | 73.9 |
Redundancy | 4.6 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |