2PSS
The structure of Plasmodium falciparum spermidine synthase in its apo-form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-11-28 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.043 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 195.630, 134.180, 48.420 |
| Unit cell angles | 90.00, 94.07, 90.00 |
Refinement procedure
| Resolution | 19.750 - 2.200 |
| R-factor | 0.20721 |
| Rwork | 0.205 |
| R-free | 0.24728 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2i7c |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.366 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.256 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.780 | 0.264 |
| Number of reflections | 59477 | |
| <I/σ(I)> | 11.59 | 5.4 |
| Completeness [%] | 94.3 | 93 |
| Redundancy | 2.26 | 2.24 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 288 | 23% w/v PEG 3350, 0.1M Ammonium Sulphate, 0.1M Bis-Tris, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 288K |
