2PRG
LIGAND-BINDING DOMAIN OF THE HUMAN PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR GAMMA
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 103 |
Detector technology | IMAGE PLATE |
Collection date | 1997-10 |
Detector | RIGAKU |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.210, 69.061, 177.966 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.300 |
R-factor | 0.207 |
Rwork | 0.207 |
R-free | 0.26400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1prg |
RMSD bond length | 0.011 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS (0.3) |
Refinement software | CNS (0.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.440 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.061 | 0.185 |
Number of reflections | 26501 | |
<I/σ(I)> | 20.4 | 4.5 |
Completeness [%] | 89.7 | 58.3 |
Redundancy | 3.42 | 2.14 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.5 | pH 7.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 25 (mM) | |
3 | 1 | drop | dithiothreitol | 5 (mM) | |
4 | 1 | drop | ammonium acetate | 500 (mM) | |
5 | 1 | reservoir | PEG4000 | 17 (%) | |
6 | 1 | reservoir | ammonium acetate | 200 (mM) | |
7 | 1 | reservoir | HEPES | 20 (mM) |