2P4A
X-ray structure of a camelid affinity matured single-domain vhh antibody fragment in complex with RNASE A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-04-17 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 70.027, 131.623, 137.156 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.900 |
R-factor | 0.18587 |
Rwork | 0.184 |
R-free | 0.21807 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2p49 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.335 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 1.900 |
Number of reflections | 48836 |
Completeness [%] | 99.1 |
Redundancy | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.5 | 295 | HAMPTON RESEARCH INDEX SCREEN, SOLUTION #67, pH 5.5, VAPOR DIFFUSION, temperature 295K |