2OPM
Human Farnesyl Diphosphate Synthase Complexed with Bisphosphonate BPH-461
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 123.2 |
Detector technology | CCD |
Collection date | 2005-09-18 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 111.783, 111.783, 66.525 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.400 |
R-factor | 0.2335 |
Rwork | 0.234 |
R-free | 0.26930 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yv5 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.016 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.100 | 0.412 |
Number of reflections | 16818 | |
<I/σ(I)> | 10.4 | |
Completeness [%] | 98.6 | 92 |
Redundancy | 12.2 | 9.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 298 | 10% PEG 2K, 0.5M Phosphate Citrate buffer, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |