2OD7
Crystal Structure of yHst2 bound to the intermediate analogue ADP-HPD, and and aceylated H4 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2005-07-15 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9777 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 106.759, 106.759, 67.689 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.920 - 2.000 |
R-factor | 0.234 |
Rwork | 0.222 |
R-free | 0.23400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1szd |
RMSD bond length | 0.007 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.064 | 0.396 |
Number of reflections | 28626 | |
<I/σ(I)> | 13.5 | 27 |
Completeness [%] | 94.0 | 94.2 |
Redundancy | 6.5 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 2.0 M Ammonium Sulfate, 0.1 M sodium citrate, 0.2 M potassium/sodium tartrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |