2NZ6
Crystal structure of the PTPRJ inactivating mutant C1239S
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-05-06 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | H 3 |
Unit cell lengths | 88.521, 88.521, 118.950 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.980 - 2.300 |
R-factor | 0.18399 |
Rwork | 0.182 |
R-free | 0.22750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2cfv |
RMSD bond length | 0.011 |
RMSD bond angle | 1.291 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.980 | 2.420 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.089 | 0.387 |
Number of reflections | 15274 | |
Completeness [%] | 99.2 | 95 |
Redundancy | 3.3 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.01M NiCl2, 0.1M TRIS, pH 8.5, 1M Li2SO4, VAPOR DIFFUSION, SITTING DROP, temperature 293K |