2NN6
Structure of the human RNA exosome composed of Rrp41, Rrp45, Rrp46, Rrp43, Mtr3, Rrp42, Csl4, Rrp4, and Rrp40
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-06-30 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.98090 |
Spacegroup name | P 4 3 2 |
Unit cell lengths | 307.800, 307.800, 307.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 14.980 - 3.350 |
R-factor | 0.291 |
Rwork | 0.291 |
R-free | 0.34400 |
Structure solution method | MIRAS |
RMSD bond length | 0.010 |
RMSD bond angle | 1.700 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 3.470 |
High resolution limit [Å] | 3.350 | 3.350 |
Rmerge | 0.080 | 0.430 |
Number of reflections | 69964 | |
<I/σ(I)> | 10.5 | 1.6 |
Completeness [%] | 97.8 | 95.5 |
Redundancy | 5.3 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 4.5-8% w/v PEG4000, 0.1M Sodium citrate, 1 mM TCEP, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |