2M3O

Structure and dynamics of a human Nedd4 WW domain-ENaC complex

Spectrometer

Experimental method: SOLUTION NMR

Spectrometer ID	Spectrometer maker	Spectrometer model	Spectrometer type	Spectrometer field strength
1	Bruker	AVANCE	Bruker Avance	600
2	Varian	Direct Drive	Varian Direct Drive	800
3	Varian	Direct Drive	Varian Direct Drive	900
4	Varian	Unity-INOVA	Varian Unity-INOVA	600

Experiment

experiment id	conditions id	solution id	Experiment type
1	1	1	2D 1H-15N HSQC
10	1	1	3D 1H-15N TOCSY
11	1	1	3D 1H-13C NOESY
12	1	2	2D CBCA(CO)NH
13	1	2	2D C(CO)NH
14	1	2	2D HNCO
15	1	2	2D HNCACB
16	1	2	3D HCCH-TOCSY
17	1	2	3D 1H-15N NOESY
18	1	2	3D 1H-15N TOCSY
19	1	2	3D 1H-13C NOESY
2	1	2	2D 1H-15N HSQC
20	1	2	2D 1H-15N NOESY
21	1	2	2D 1H-13C NOESY
22	1	2	3D 15N/13C-EDITED NOESY
23	1	2	2D 13C-edited/filtered NOESY
24	1	1	2D long-range HNCO
25	1	3	2D 1H-15N R2 relaxation
26	1	3	2D 1H-15N R1 relaxation
27	1	3	2D 1H-15N heteronuclear NOE
28	1	3	2D 1H-15N R1rho relaxation
29	1	3	2D 1H-15N R1 relaxation
3	1	1	3D CBCA(CO)NH
30	1	3	2D 1H-15N heteronuclear NOE
31	1	3	2D 1H-15N R1rho relaxation
32	1	3	2D 1H-15N R1 relaxation
33	1	3	2D 1H-15N heteronuclear NOE
34	1	1	3D HCAN
35	1	2	2D HCAN
36	1	1	2D HBCBCGCDHD
37	1	1	2D HBCBCGCDCEHE
38	1	2	2D HBCBCACONHAN
39	1	2	2D HBCBCACONHACA
4	1	1	3D C(CO)NH
40	1	3	2D 15N CPMG dispersion
41	1	3	2D 15N CPMG dispersion
5	1	1	3D HNCO
6	1	1	3D HNCACB
7	1	1	3D H(CCO)NH
8	1	1	3D HCCH-TOCSY
9	1	1	3D 1H-15N NOESY

NMR Sample

conditions id	NMR sample pH	NMR sample pressure	NMR sample temperature
1	6.5	ambient	298

Conformers

Conformers Calculated Total Number	200
Conformers Submitted Total Number	15