2JCS
Active site mutant of dNK from D. melanogaster with dTTP bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.523, 119.467, 68.900 |
| Unit cell angles | 90.00, 92.42, 90.00 |
Refinement procedure
| Resolution | 32.280 - 2.500 |
| R-factor | 0.232 |
| Rwork | 0.232 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oe0 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.885 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.280 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.090 | 0.310 |
| Number of reflections | 18797 | |
| <I/σ(I)> | 6.9 | 2.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.12 M NAAC,0.1 M MES PH 6.5, 18% MPEG 2000 |
