2JBU
Crystal structure of human insulin degrading enzyme complexed with co- purified peptides.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-07-30 |
Detector | ADSC CCD |
Spacegroup name | P 65 |
Unit cell lengths | 263.277, 263.277, 90.735 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.860 - 3.000 |
R-factor | 0.186 |
Rwork | 0.186 |
R-free | 0.24000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2g47 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.500 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.120 | 0.500 |
Number of reflections | 72886 | |
<I/σ(I)> | 19.9 | 4.2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 11.4 | 11.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |