2I6B
Human Adenosine Kinase in Complex with An Acetylinic Inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 110 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.180, 58.200, 89.210 |
| Unit cell angles | 90.00, 107.54, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.300 |
| Rwork | 0.221 |
| R-free | 0.29100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2i6a |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | BUSTER |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.300 |
| Rmerge | 0.068 |
| Number of reflections | 25799 |
| Completeness [%] | 87.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | PEG4000, 0.1 M MgCl2, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
