2GVD

Complex Of Gs- With The Catalytic Domains Of Mammalian Adenylyl Cyclase: Complex With TNP-ATP and Mn

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-10-24
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0781
Spacegroup name	P 21 21 2
Unit cell lengths	118.200, 133.400, 70.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.960 - 2.900
R-factor	0.245
Rwork	0.245
R-free	0.27900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1tl7
RMSD bond length	0.010
RMSD bond angle	0.900
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	15.000
High resolution limit [Å]	2.900
Number of reflections	22450
Redundancy	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	289	7.5-7.8% PEG8000, 0.5M NACL, 0.1M PHOSPHATE BUFFER, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 289K