2GVD

Complex Of Gs- With The Catalytic Domains Of Mammalian Adenylyl Cyclase: Complex With TNP-ATP and Mn

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Adenylate cyclase type 5	polymer	225	25526.5	1	UniProt (P30803) Pfam (PF00211)	Canis lupus familiaris (dog)	Adenylate cyclase type V, ATP pyrophosphate-lyase 5, Adenylyl cyclase 5, Ca2+, -inhibitable adenylyl cyclase
2	B (B)	Adenylate cyclase type 2	polymer	212	23717.0	1	UniProt (P26769) Pfam (PF00211)	Rattus norvegicus (Norway rat)	Adenylate cyclase type II, ATP pyrophosphate-lyase 2, Adenylyl cyclase 2
3	C (C)	Guanine nucleotide-binding protein G(s), alpha subunit	polymer	394	45769.5	1	UniProt (P04896) Pfam (PF00503)	Bos taurus (cattle)	Adenylate cyclase-stimulating G alpha protein
4	D, E, H (A, C)	MANGANESE (II) ION	non-polymer		54.9	3	Chemie (MN)
5	F (A)	METHYLPIPERAZINOFORSKOLIN	non-polymer		550.7	1	Chemie (FKP)
6	G (A)	SPIRO(2,4,6-TRINITROBENZENE[1,2A]-2O',3O'-METHYLENE-ADENINE-TRIPHOSPHATE	non-polymer		718.3	1	Chemie (128)
7	I (C)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
8	J (C)	5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE	non-polymer		539.2	1	Chemie (GSP)
9	K, L, M (A, B, C)	water	water		18.0	51	Chemie (HOH)

Sequence modifications

A: 363 - 580 (UniProt: P30803)

PDB	External Database	Details
Met 476	Val 557	engineered mutation
Met 356	-	initiating methionine
His 357	-	expression tag
His 358	-	expression tag
His 359	-	expression tag
His 360	-	expression tag
His 361	-	expression tag
His 362	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	95013.1
Non-Polymers*	Number of molecules	7
	Total formula weight	2008.5
All*	Total formula weight	97021.6

*Water molecules are not included.