2G1T
A Src-like Inactive Conformation in the Abl Tyrosine Kinase Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 100 |
| Wavelength(s) | 0.972425 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.586, 78.440, 141.609 |
| Unit cell angles | 90.00, 90.11, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.800 |
| R-factor | 0.2208 |
| Rwork | 0.219 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1m52 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.295 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.890 |
| High resolution limit [Å] | 1.800 | 1.820 |
| Number of reflections | 117589 | |
| Completeness [%] | 99.8 | 85.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.2M Sodium Acetate, 100mM Sodium Cacodylate pH 6.5, 25% PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
