2FSA
Crystal structure of PHD finger-linker-bromodomain fragment of human BPTF in the H3(1-15)K4ME2 bound state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9639 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.451, 85.169, 109.567 |
| Unit cell angles | 90.00, 99.03, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| Rwork | 0.200 |
| R-free | 0.22000 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2f6j |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.032 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.070 | 0.343 |
| Number of reflections | 54370 | |
| <I/σ(I)> | 20.2 | 2.68 |
| Completeness [%] | 98.6 | 98.3 |
| Redundancy | 3 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 17% PEG 4000, 8.5% ISO-PROPANOL, 0.085M HEPES-NA, 15% GLYCEROL ANHYDROUS , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
