2FSA

Crystal structure of PHD finger-linker-bromodomain fragment of human BPTF in the H3(1-15)K4ME2 bound state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2005-12-15
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9639
Spacegroup name	P 1 21 1
Unit cell lengths	38.451, 85.169, 109.567
Unit cell angles	90.00, 99.03, 90.00

Refinement procedure

Resolution	20.000 - 1.900
Rwork	0.200
R-free	0.22000
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	2f6j
RMSD bond length	0.005
RMSD bond angle	1.032
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.070	0.343
Number of reflections	54370
<I/σ(I)>	20.2	2.68
Completeness [%]	98.6	98.3
Redundancy	3	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	17% PEG 4000, 8.5% ISO-PROPANOL, 0.085M HEPES-NA, 15% GLYCEROL ANHYDROUS , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K